![]() ![]() Studying the role of NS involves visualizing them with a fluorescently tagged factor that localizes to NS, or the use of antibodies that show specific staining of NS. ![]() ![]() NS have been shown to be involved in replication of herpes simplex virus ( Chang et al., 2011), processing and trafficking of Influenza A virus mRNA ( Mor et al., 2016), detaining repetitive RNA originating from the transcription of repeat expanded loci that trigger Huntington's disease, spinocerebellar ataxia and dentatorubral–pallidoluysian atrophy ( Urbanek et al., 2016), but also repetitive RNA from artificial constructs that produce RNA capable of phase-separation in vitro ( Jain and Vale, 2017). Despite their prevalence, the function of NS remains largely unknown, although they have been proposed to act as reservoirs for splicing factors, and association with NS have been shown to correlate with enhanced transcription and RNA-processing ( Chen and Belmont, 2019 Galganski et al., 2017). Under normal conditions, they appear as irregularly shaped, dynamic structures that show hallmarks of phase-separated condensates, such as fusion and deformation under pressure in living cells ( Chen and Belmont, 2019 Zhang et al., 2016). Nuclear speckles (NS) are membraneless nuclear bodies ( Banani et al., 2017 Shin and Brangwynne, 2017) in the interchromatin-space of the nucleus that contain high concentrations of RNA-processing and some transcription factors but are devoid of DNA ( Spector and Lamond, 2011). These newly discovered core proteins could therefore further our understanding of the role NS play in disease. Nuclear speckles have been associated with certain viral infections, and seem to help prevent the onset of diseases such as Huntington’s and spinocerebellar ataxia. This suggests that SRMM2 and SON make up the scaffold that holds the proteins in NS together. found that depleting SON and SRRM2 from human cells caused other proteins associated with the NS to diffuse away from their territories and become dispersed within the nucleus. Studying the evolutionary history of SRRM2 led to the identification of another protein with similar properties called SON. discovered that although the structure and sequence of SRMM2 varies between different animal species, a small region of this protein remained unchanged throughout evolution. The analysis revealed that SC35 attaches to certain parts of a large, flexible protein called SRRM2. ![]() studied NS in human cells grown in the lab. However, it was unclear which parts of the NS this marker binds to. Typically, researchers visualize NS using a substance called SC35 which targets specific sites in these structures. Although NS were discovered over a century ago, little is known about their scaffold proteins, making it difficult to understand the precise role of these speckles. Structures like NS often contain a number of different factors held together by a core group of proteins known as a scaffold. One of the first nuclear territories to be identified were granular looking structures named Nuclear Speckles (or NS for short), which are thought to help process RNA before it leaves the nucleus. However, the contents of the nucleus are not randomly arranged, and these proteins are often clustered into specialized areas where they perform their designated roles. Inside the nucleus, DNA is tightly packed together with proteins that can read the cell’s genetic code and convert into the RNA molecules needed to build proteins. Most cells store their genetic material inside a compartment called the nucleus, which helps to separate DNA from other molecules in the cell. ![]()
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Lomax/Lomax-AmericanBalladsAndFolkSongs, pp. Lomax/Lomax-FolkSongUSA 76, "Paddy Works On the Erie" (1 text, 1 tune) Williams-Wiltshire-WSRO Mi 670, "Paddy Works on the Railway" (1 text) 20-21, "Poor Paddy Works on the Railway" (1 conflated text, 1 tune) MacColl-ShuttleAndCage-IndustrialFolkBallads, pp. 77-78, "Paddy Works on the Railway" (1 text, 1 tune) 337-338, "Paddy Works on the Railway," "Poor Paddy Works on the Railway" (2 texts, 2 tunes) 155-156, "Roll the Cotton Down" (1 text, version "E" of "Roll the Cotton Down") pp. 139-141, "Oh, Poor Paddy Works on the Railway" (1 text, 1 tune) 107-108, "Paddy Works on the Railway" (1 text, 1 tune) 356-357, "Poor Paddy Works on the Railway" (1 text, 1 tune)Ĭolcord-SongsOfAmericanSailormen, pp. 547-552, "Paddy Works on the Railway" (1 text plus extended excerpts to illustrate variations in the song and a broadside print of "Mick Upon the Railroad," 1 tune) KEYWORDS: railroading work marriage death drink hardtimesĬohen-LongSteelRail, pp. to work upon the (railway)." 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A genetic variation in the adenosine A2A receptor gene (ADORA2A) contributes to individual sensitivity to caffeine effects on sleep. Rétey JV, Adam M, Khatami R, Luhmann UF, Jung HH, Berger W, et al. A behavioral perspective on insomnia treatment. The relationship between insomnia and body temperatures. Lack LC, Gradisar M, Van Someren EJ, Wright HR, Lushington K. Chronic insomnia is associated with nyctohemeral activation of the hypothalamic-pituitary-adrenal axis: clinical implications. Vgontzas AN, Bixler EO, Lin HM, Prolo P, Mastorakos G, Vela-Bueno A, et al. Caffeine use as a model of acute and chronic insomnia. 24-Hour metabolic rate in insomniacs and matched normal sleepers. Functional neuroimaging evidence for hyperarousal in insomnia. Nofzinger EA, Buysse DJ, Germain A, Price JC, Miewald JM, Kupfer DJ. Predisposition in the Evolution of Insomnia: Evidence, Potential Mechanisms, and Future Directions. Principles and Practice of Sleep Medicine. Pharmacological Treatment: Other Medications. GABAA receptors: subtypes, regional distribution, and function. Sleep circuitry and the hypnotic mechanism of GABAA drugs. Hypocretins (orexins) and sleep-wake disorders. The sleep switch: hypothalamic control of sleep and wakefulness. Hypothalamic regulation of sleep and circadian rhythms. Melatonin in the regulation of sleep and circadian rhythms. Psychological and behavioral treatment of insomnia:update of the recent evidence (1998-2004). Morin CM, Bootzin RR, Buysse DJ, Edinger JD, Espie CA, Lichstein KL. Cognitive behavior therapy for chronic insomnia occurring within the context of medical and psychiatric disorders. National Institutes of Health State of the Science Conference statement on Manifestations and Management of Chronic Insomnia in Adults, June 13-15, 2005. The economic burden of insomnia: direct and indirect costs for individuals with insomnia syndrome, insomnia symptoms, and good sleepers. 22 Suppl 2:S379-85.ĭaley M, Morin CM, LeBlanc M, Grégoire JP, Savard J. Zammit GK, Weiner J, Damato N, Sillup GP, McMillan CA. Psychomotor performance deficits and their relation to prior nights' sleep among individuals with primary insomnia. Arlington, VA: American Psychiatric Association 2013.Įdinger JD, Means MK, Carney CE, Krystal AD. Diagnostic and Statistical Manual of Mental Disorders. Princeton, NJ,: The Gallup Organization 1979.Īmerican Psychiatric Association. Sleep Disorders Medicine: Basic Science, Technical Considerations, and Clinical Aspects, 3rd edition, p361. Cambridge University Press, Cambridge UK, 2003, et al.Ĭhokroverty S. Diagnosis and Classification of Insomnia Disorders. Westchester, Ill: American Academy of Sleep Medicine 2005. ICSD2 - International Classification of Sleep Disorders. An american academy of sleep medicine report. ![]() Practice parameters for the psychological and behavioral treatment of insomnia: an update. Morgenthaler T, Kramer M, Alessi C, Friedman L, Boehlecke B, Brown T, et al. ![]() Standards of Practice Committee of the American Academy of Sleep Medicine. An American Academy of Sleep Medicine report. ![]() Practice parameters for the nonpharmacologic treatment of chronic insomnia. 25(5):539-58.Ĭhesson AL Jr, Anderson WM, Littner M, Davila D, Hartse K, Johnson S, et al. Cognitive-behavioral therapy for primary insomnia. Clinical Practice Guideline for the Pharmacologic Treatment of Chronic Insomnia in Adults: An American Academy of Sleep Medicine Clinical Practice Guideline. Sateia MJ, Buysse DJ, Krystal AD, Neubauer DN, Heald JL. Montagna P, Gambetti P, Cortelli P, Lugaresi E. Clinical guideline for the evaluation and management of chronic insomnia in adults. Schutte-Rodin S, Broch L, Buysse D, Dorsey C, Sateia M. ![]() ![]() ![]() Most percussion instruments as the term is normally understood are classified as idiophones and membranophones. ![]() The Hornbostel–Sachs system has no high-level section for percussion. In a musical context then, the percussion instruments may have been originally coined to describe a family of musical instruments including drums, rattles, metal plates, or blocks that musicians beat or struck to produce sound. However, all known uses of percussion appear to share a similar lineage beginning with the original Latin percussus. The term is not unique to music, but has application in medicine and weaponry, as in percussion cap. As a noun in contemporary English, Wiktionary describes it as the collision of two bodies to produce a sound. The word percussion derives from the Latin verb percussio to beat, strike in the musical sense, and the noun percussus, a beating. Percussion instruments are classified by various criteria sometimes depending on their construction, ethnic origin, function within musical theory and orchestration, or their relative prevalence in common knowledge. Usually started to be played at a younger age for more talented individuals, commonly started at age 10 - 12 Rhythm, melody, and harmony are all represented in these ensembles. In more recent popular-music culture, it is almost impossible to name three or four rock, hip-hop, rap, funk or even soul charts or songs that do not have some sort of percussive beat keeping the tune in time.īecause of the diversity of percussive instruments, it is not uncommon to find large musical ensembles composed entirely of percussion. In classic jazz, one almost immediately thinks of the distinctive rhythm of the hi-hats or the ride cymbal when the word-swing is spoken. In military marching bands and pipes and drums, it is the beat of the bass drum that keeps the soldiers in step and at a regular speed, and it is the snare that provides that crisp, decisive air to the tune of a regiment. ![]() In almost every style of music, percussion plays a pivotal role. The use of percussion instruments became more frequent in the 20th century classical music. In the 18th and 19th centuries, other percussion instruments (like the triangle or cymbals) have been used, again generally sparingly. Rather, they serve to provide additional accents when needed. However, often at least one pair of timpani is included, though they rarely play continuously. Most classical pieces written for full orchestra since the time of Haydn and Mozart are orchestrated to place emphasis on the strings, woodwinds, and brass. In jazz and other popular music ensembles, the pianist, bassist, drummer and sometimes the guitarist are referred to as the rhythm section. Percussion is commonly referred to as "the backbone" or "the heartbeat" of a musical ensemble, often working in close collaboration with bass instruments, when present. Percussion instruments may play not only rhythm, but also melody and harmony. ![]() Percussion instruments are most commonly divided into two classes: pitched percussion instruments, which produce notes with an identifiable pitch, and unpitched percussion instruments, which produce notes or sounds in an indefinite pitch. On the other hand, keyboard instruments, such as the celesta, are not normally part of the percussion section, but keyboard percussion instruments such as the glockenspiel and xylophone (which do not have piano keyboards) are included. Percussive techniques can even be applied to the human body itself, as in body percussion. However, the section can also contain aerophones, such as whistles and sirens, or a blown conch shell. The percussion section of an orchestra most commonly contains instruments such as the timpani, snare drum, bass drum, tambourine, belonging to the membranophones, and cymbals and triangle, which are idiophones. It is shown below that percussion instruments may belong to the organological classes of ideophone, membranophone, aerophone and cordophone. In spite of being a very common term to designate instruments, and to relate them to their players, the percussionists, percussion is not a systematic classificatory category of instruments, as described by the scientific field of organology. Excluding zoomusicological instruments and the human voice, the percussion family is believed to include the oldest musical instruments. A percussion instrument is a musical instrument that is sounded by being struck or scraped by a beater including attached or enclosed beaters or rattles struck, scraped or rubbed by hand or struck against another similar instrument. ![]() ![]() ![]() By using Snapgene’s “Import primers from a list” function, we can detect all of the primers we have designed that will anneal to the plasmid sequence. We have designed many primers for Sanger sequencing at Addgene. If a plasmid contains a region with a high amount of guanine and cytosine nucleotides, we might need to send it for Sanger sequencing to verify the sequence within that region. These regions require additional verification experiments that can be partially designed using the Snapgene desktop software. These can include GC-rich and repetitive regions. Some regions of plasmids are difficult to sequence by NGS. 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To design experiments for further plasmid verification.To align NGS sequencing results with reference sequences.The main ways we use SnapGene for plasmid quality control are: In this blog, we’ll walk you through how a scientist at Addgene uses Snapgene to confirm the sequence of a plasmid and we’ll highlight some of the new features available on our website through our Snapgene powered maps and sequence analysis tools. We regularly use Snapgene for our quality control process because of its expansive feature library and useful tools. We’ve also talked about how our new Snapgene generated maps provide improved feature detection with an easy to use interface. We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell and going into detail about how we use next generation sequencing results to perform quality control on deposited plasmids. ![]() |
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